Unexpected detection of Plasmodium vivax and Plasmodium falciparum DNA in asymptomatic blood donors: fact or artifact?

A study searching for Plasmodium vivax and Plasmodium falciparum DNA among blood donors from the non-endemic area in Brazil reported a rate of 7.41%. This number is at least three times higher than what has been observed in blood donors from the Amazon, an endemic area concentrating >99% of all malaria cases in Brazil. Moreover, the majority of the donors were supposedly infected by P. falciparum, a rare finding both in men and anophelines from the Atlantic forest. These findings shall be taken with caution since they disagree with several publications in the literature and possibly overestimate the actual risk of malaria transmission by blood transfusion in São Paulo city.

last two decades, several studies focused on various aspects of "bromeliad" malaria transmission [10,11]. In some of those investigations, DNA supposed to be from P. falciparum was also amplified [10], but it did not usually resist to a second amplification by another method. Sometimes, the second amplification disclosed P. malariae, instead.
So far there are only two published studies investigating Plasmodium prevalence by molecular methods among Brazilian blood donors [12,13], both performed in the endemic area (the Amazon) and cited in the paper of Maselli et al. [1]. The former described a 2.3% prevalence of P. vivax + P. falciparum infections, while the latter detected 1.34%. More than 99.9% of the reported Brazilian malaria cases come from this area so, obviously, more asymptomatic cases may occur there as well. How would this data conciliate with the 7.41% prevalence in São Paulo city and surroundings, as reported by Maselli  whereas the primers employed in their real-time PCR assay are species-specific and most probably taken from the description of Perandin et al. [18], although there are a few nucleotide differences in the FAL-F and VIV-R primers.
It may be argued that the actual contribution of donors included in the "exposed" group to the total blood donors pool from Fundação Pró-Sangue/Hemocentro de São Paulo (FPS) is much less than the approximately 45% (500/1,108) enrolled in the study. This would justify a verified much lower rate of TTM than those numbers would predict. However, in an analysis of 17 spontaneous blood donors from Juquitiba (Atlantic Forest area, São Paulo state, one of the cities that directed donors to the "exposed" group in Maselli's et al. work) that came to FPS, the ELISA using P. vivax recombinant antigen (ELISA-Pv) was reagent in 76% of samples and using P. falciparum total extract in 12%. SD Bioline test using recombinant antigens for both species (SD Bioline Pf/Pv) resulted positive in 71% for P. vivax and 0% for P. falciparum. This result points to a cross-reaction with P. vivax antibodies using P. falciparum total extract. One sample was positive for P. malariae by PCR [19]. Recently, samples of 39 donors from the same area were assayed employing a modified real-time PCR protocol [20] using genus-specific primers [17]. ELISA-Pv was reagent in 38% of samples and SD Bioline Pf/Pv was positive in 38% (Pv band) and 0% (Pf band). No sample was positive in real-time PCR (data not published).
The PhD thesis linked to this study is freely available [21] and it reports that ELISA was performed with the 84 samples found DNA+. Only eleven out of the 61 samples Plasmodium DNA + from the "exposed" group were seropositive, while none of the 23 from the non-exposed group was positive. This data is not included in the published manuscript. This huge discrepancy was never described in other cohorts. For example, 100% of blood donors in Nigeria were seropositive for P. falciparum [22], so it remains to be explained why in the São Paulo state cohort, donors that supposedly carry P. falciparum or P. vivax do not develop detectable antibodies.
The sample size was calculated based on the 1.3% prevalence of subclinical infection in blood donors in the Amazon region. However, this data is not available in the cited reference (ref.

Conclusions
In conclusion, undoubtedly the issue of TTM in São Paulo State deserves further investigation, and certainly, there is an enhanced risk from donors with some epidemiological connection to the forested areas where Plasmodium (mainly P. malariae and P. vivax) persists, since currently there are no active measures to identify such asymptomatic carriers. Unfortunately, the work by Maselli et al., instead of helping in clarifying the size of the risk and potential epidemiological associations in infected donors, brings confusion and unjustified alarm by presenting unrealistic data of donors carrying Plasmodium DNA.

Competing interests
The authors declare that they have no competing interests.
Authors' contributions CCJ and RSM reviewed the entomological aspects of Malaria in the Atlantic Forest. MB and SMDS reviewed the general epidemiology of Plasmodium infections in patients from São Paulo state. MCAS analysed the serological results. AMJ, JEL and VO evaluated the data from blood donors. All authors read and approved the final manuscript.