Figure 3

A representative multi-color flow cytometry gating strategy to quantify different B cell subsets in Kisumu and Nandi infants. The B cell subsets FACS plots are of a representative Kisumu and Nandi infant. Within the live CD19+ B cell gate, the B cell subsets were discriminated as follows: immature transitional B cells (CD19+CD10+CD34-), classical naive B cells (CD19+IgD+CD27-), classical memory cells (CD19+IgD-CD27+), non class-switched memory B cells (CD19+IgD+CD27+) and CD19+IgD-CD27- B cells.