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Table 2 Comparative flow cytometric analysis of peripheral lymphocytes distribution in infants from Kisumu and Nandi

From: Suppression of circulating IgD+CD27+ memory B cells in infants living in a malaria-endemic region of Kenya

 

Kisumu

 

Nandi

 

Parameter

12 months

18 months

24 months

p- value

12 months

18 months

24 months

p -value

CD45+CD19+ B

17.55

18.93

19.61

0.4233

14.80

15.81

15.77

0.8372

cells

(15.03-28.35)

(17.05-24.41)

(15.23-22.44)

 

(10.65-21.45)

(11.72-19.02)

(12.79-18.95)

 

CD45+CD3+ T

66.35

66.94

69.62

0.0768

73.80

74.44

71.79

0.9995

cells

(59.23-70.35)

(62.42-73.87)

(66.15-75.10)

 

(67.70-79.45)

(68.56-76.79)

(69.47-77.74)

 

CD3+CD4+ T

58.30

63.72

65.12

0.2275

70.40

62.31

61.53

0.1315

cells

(54.15-67.10)

(58.19-70.84)

(49.09-72.07)

 

(60.50-74.50)

(57.26-72.63)

(53.97-67.18)

 

CD3+CD8+ T

32.70

25.81

26.33

0.1684

24.40

27.06

31.20

0.2144

cells

(23.85-36.75)

(21.99-34.05)

(21.58-44.75)

 

(19.75-31.65)

(22.06-35.69)

(25.53-33.70)

 

CD4/CD8 ratio

2.18(0.27)

2.61(0.33)

2.18(0.32)

0.5280

2.83(0.27)

2.67(0.35)

2.13(0.20)

0.0571

  1. Freshly isolated PBMCs from Kisumu (n = 24) and Nandi (n = 21) infants were analyzed by multicolor flow cytometry following staining with various combinations of monoclonal antibodies (mAB). The data are presented as median (25th-75th percentiles) of the lymphocyte subset analyzed unless otherwise stated. Statistical differences between the two cohorts was determined by Mann-Whitney U test while age-related changes in lymphocyte subset within cohorts was analyzed using Friedman test with post-hoc Dunns test for multiple comparisons. Statistically significant p- values of ≤0.05 are in bold