Genetic diversity of expressed Plasmodium falciparum var genes from Tanzanian children with severe malaria

Malaria Journal

Table 3 Summary of analysed sequences of different transcribed var DBL1α sequences

Isolates	RT-PCR, cloned in pGEMT Vector, PCR screened 96 clones picked for sequencing	Sequences generated per isolate	Number of distinct DBLα *var* per isolate	Predominant gene blasted vs 3D7	Group homology to 3D7	Bulls’ signature and group	MOI
Severe
ISM 2	48	42	23	PF08_0141	A	LFLG-IREY-KAIT-2-LTNL	2
ISM 3	48	41	22	PFF0010w	B/A	LYLD-FREY-KAIT-2-PTNL	3
ISM 11	60	50	20	PFD1005c	B/C	LFIG-LRED-KALT-4-PTYF	2
ISM 16	48	36	18	PFD0020c	A	MFKR-LRED-RAIT-2-PTNL	1
ISM 33	60	50	15	PFF0010w	A	LFLG-VREY-KAIT-2-LTNL	3
ISM 48	48	47	12	PF08_0141	A	MFLG-IREY-KALT-2-PTNL	3
ISM 49	48	44	17	PFL1830c	B	LYLG-LRED-KAIT-4-PTYF	3
Asymptomatic
IAM 5	48	46	25	PF08_0141	A	MFLG-IREY-KALT-2-PTNL	3
IAM 7	48	45	8	PFL 1830c	B	LYLG-LRED-KALT-4-PTYF	2
IAM 10	48	38	23	PFD0615c	C	LFIG-LRED-EAIT-4-PTNF	3
IAM 11	48	43	15	PFL2665c	B	LYRG-LRED-NAII-3-LTNF	3
IAM 12	48	45	20	PFL1955w	B/C	LYLG-LRED-KAIT-4-PTYF	2
IAM 17	48	46	24	PFA0005w	B	LYLG-LRED-EAIT-4-PTYF	2
IAM 18	48	42	16	PFA0005w	B	LYLG-LRED-KAIT-4-PTYF	2
	Total	615

ISSN: 1475-2875