Effect of ingested human antibodies induced by RTS, S/AS01 malaria vaccination in children on Plasmodium falciparum oocyst formation and sporogony in mosquitoes

Table 2 Transmission-blocking activities of human polyclonal IgGs fed three days post-infected blood meal ^a

Sample	Mean ooc^b	PIm[o]^c	p-value^d	Mosquito^e	PIp[o]^f	p-value^g	Mean Spz^h	PIm[s]ⁱ
CS-1	30.0	1	0.93	3/20	13	0.07	71.3	-184
	(0, 75)	(-148, 62)			(-1, 35)
CS-2	29.9	1	0.92	0/20	-3	0.48	49.4	-97
	(2, 56)	(-139, 62)			(-15, 14)
CS-3	37.6	-24	0.66	0/20	-3	0.48	56.9	-127
	(5, 69)	(-190, 51)			(-15, 14)
CS-4	34.5	-14	0.88	3/20	13	0.07	35.4	-41
	(0, 87)	(-175, 57)			(-1, 35)
Contl-1	25.5	N/A	N/A	0/20	N/A	N/A	43.1	N/A
	(1, 80)
Contl-2	35.0	N/A	N/A	1/20	N/A	N/A	7.0	N/A
	(0, 83)

^aAll purified IgGs were tested at 3.75 mg/ml and fed three days post-infected blood meal.
^bArithmetic mean (range) of oocysts per mosquito midgut.
^cPer cent inhibition of mean oocyst intensity (PIm[o]) and the 95% confidence interval (95% CI).
^dTwo-sided p-values for testing whether PIm[o] is significantly different from 0.
^eNumber of mosquitoes without any oocysts/number of mosquitoes examined.
^fPer cent inhibition of prevalence of mosquitoes with oocysts (PIp[o]) and the 95% CI.
^gp-values of PIp[o].
^hNumber of sporozoites (×10³) per mosquito calculated from a pool of salivary glands of 12 mosquitoes.
ⁱPer cent inhibition of mean sporozoite intensity (PIm[s]) calculated with the average of sporozoites counts of Contl-1 and Contl-2.

ISSN: 1475-2875