Figure 2From: Production and characterization of specific monoclonal antibodies binding the Plasmodium falciparum diagnostic biomarker, histidine-rich protein 2Analysis of purified recombinant mAbs D2 and F9 on 4-12% SDS-PAGE. The gel was stained with Coomassie Blue R250 (A). Lane 1: SeeBlue Plus2 Pre-Stained Standard (Invitrogen); Lanes 2 and 3: non-reduced mAb D2; Lanes 4 and 5: non-reduced mAb F9; Lanes 6 and 7: reduced mAb D2; Lanes 8 and 9: reduced mAb F9, at neat, and 1 in 10 dilutions. Analytical size exclusion chromatography of protein-A purified recombinant mAbs D2 and F9 at 280Â nm are shown in panels B and C, respectively.Back to article page