Figure 4

Western blot analysis for the binding specificity of mAbs against recombinant and native PfHRP2 proteins. A: Separation of recombinant PfHRP2 and 1% BSA proteins in 10% reduced SDS-PAGE, and stained with Coomassie Blue; B: Hybridization of corresponding recombinant mAbs D2, F9, and C1-13 to rPfHRP2 (1 μg/mL) and 1% BSA in Western blot. C: Separation of native malaria parasite protein and uninfected RBC by10% SDS-PAGE, stained with Coomassie Blue. D: hybridization of corresponding recombinant mAbs D2, F9, and C1-13 to malaria native protein (1 μg/mL) and uninfected RBC protein (1 μg/mL)in Western blot. Lane 1: recombinant HRP2 protein; Lane 2: negative control 1% BSA. Lane 3: P. falciparum-infected RBC protein; Lane 4: negative control uninfected RBC protein. Molecular weights are shown at the left corner of the diagram.