Figure 2From: Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysisReaction sensitivity of the recombinase polymerase amplification. A serial dilution of genomic DNA from P. falciparum 3D7 was used to test the analytical sensitivity of the assay. Positive RPA reaction products (174Â bp) can be detect on a stained agarose gel (2%) (A). In the lateral flow format (LF-RPA) the sensitivity was 100Â fg of genomic DNA (B). NTC: no template controls contained water.Back to article page