Figure 3From: Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysisDetermination of reaction temperature and time. The LF-RPA works effectively in a broad range of constant reaction temperatures (A). After 10Â min of isothermal amplification reaction, the test line is visible on the test strip. Including the incubation of 5Â min the whole assay time of the LF-RPA is less than 20Â min (B).Back to article page