Figure 1

A general model of ookinete entry into the mosquito midgut epithelium. (a) Ookinetes (shown in green) enter the apical surface of the midgut epithelium, through the microvillar brush border (MV), where the lateral membranes (LM) of adjacent epithelial cells (EC) converge [47,51,52]. (b-c) Ookinete movement into the midgut epithelium causes substantial localized invagination of the latter (indicated by small blue arrows) [52,57]. (d) Ookinetes subsequently enter midgut epithelial cells through the lateral apical membrane immediately adjacent to the site where the intercellular junctions (IJ) begin [47,51,52]. (e) The ookinete proceeds intracellularly towards the basal membrane (BM) of the invaded midgut epithelial cell which exhibits morphological abnormalities including protrusion (indicated by large black arrow) into the midgut lumen (LUM) [44–48,52,54,55].