Figure 3

Evolution of parasitaemia monitored by double staining flow cytometry in subcultures done from the two flasks in which surviving parasites were observed at day 36 in the main pressure experiment. Arrows indicate the date in the main experiment at which surviving parasites were detected on Giemsa smears and confirmed by flow cytometry. A: Evolution in subcultures done under a continuous 100 nM FQ pressure. B: Evolution in subcultures done in the absence of FQ pressure.