Figure 2

Entire P. vivax intergenic regions are poorly recognized by P. falciparum. A. Schematic representation of reporter plasmids with entire intergenic regions. EF-1α IR – elongation factor one alpha intergenic region; Pvvir24 IR – intergenic region between P. vivax variant genes 23 and 24; Pv – P. vivax; Pf – P. falciparum; Pb – P. berghei LUC – luciferase; DT3' – P. berghei dihydrofolate reductase 3' UTR. B. Transient transfections in P. falciparum. Plasmids were transiently transfected in P. falciparum and luciferase activity determined 4 days later. Luciferase expression is represented relative to the activity of pE(A)b.luc.^D. Negative control (-) refers to luminescence measures of the substrate alone or plasmid with luciferase and malaria 3' UTR but no promoter. Log scale was used. Values represent the mean of at least three independent experiments done with two or three different DNA preparations. Bars represent standard deviations.