Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants

Table 1 Primers and probes selected for PCR of Plasmodium 18S rRNA and cox1 genes

			Sequence	Target gene
Primers	Genus specific	Forward	5'-GTTTAAGGCAACAACAGGT-3'	18S rRNA
		Reverse	5'-CAATAATCTATCCCCATCACGA-3'
	P. falciparum specific	Forward	5-TTACATCAGGAATGTTATTGC-3	cox1
		Reverse	5-ATATTGGATCTCCTGCAAAT-3
Probes		Sensor	5'-ACTCGTTATACATATCAGTGTAGCACGC (FLU)-3'	18S rRNA
			5'-(Red 705)
		Anchor	GCAGCCTAGTTCATCTAAGGACATCACAG (P)- 3'

Amplicon length: P. ovale: 198 pb (1662 – 1859); P. vivax: 178 pb (1435 – 1612); P. malariae: 207 pb (1873 – 2079) and P. falciparum: 190 pb (1035 – 1225). The nucleotide positions are those reported in GenBank

ISSN: 1475-2875