Table 1 Primers for amplification and sequencing of Pvdhfr-ts and flanking, using PCR product A (amplifies P. vivax dhfr-ts and 792 bp upstream, 683 bp downstream). All sequences are written 5' to 3'
From: Multiple origins of resistance-conferring mutations in Plasmodium vivax dihydrofolate reductase
Primer name | Use | Sequence |
|---|---|---|
S1048 | PCR A 1st nested | TGA ACA GCC AAG CGA ATA GGT AAA A |
S1049 | PCR A 1st nested | ATT TGA AGG TTA AAA TGG GGT GAC G |
S1105 | PCR A 2nd nested/sequencing | ATG CAC ACA TCT TCG CTC TC |
S1106 | PCR A 2nd nested/sequencing | TCT TTT TCA CGT TCG CTG TG |
S303 | Sequencing | ATG GAG GAC CTT TCA GAT GTA TT |
S1090 | Sequencing | AAA AAA ATA TGC ACT CCC CAT TT |
S1092 | Sequencing | ACT TTT ATA GCT AGC TAG CGA AGT GTT |
S1166 | Sequencing | TTT TCT TCG CGG CGA CAA |
S1088 | Sequencing | CAG TTA TAT GCA CAC ATC TTC GC |
S1095 | Sequencing | ACT GCG GAC AGC GCT TCG |
S1096 | Sequencing | TGA AGA TTA AGC AGC ACC CAG |
S1098 | Sequencing | ATG GCT TTA CCT CCT TGT CA |