Table 2 Sequences of oligonucleotide primers for Real Time PCR analysis of GIMAP s
Name | Accession # | Forward primer | Reverse primer |
|---|---|---|---|
Internal control genes | |||
6PFKc | Y19008 | 5'-GAGTGGAGC | 5'-CACGTTGAGGTA |
| Â | Â | GGACTTCTGGA-3' | GGAATACTTCTGC-3' |
Cirhin | BC027399 | 5'-ATTACGATGC | 5'-CCACAGTTCCAA |
| Â | Â | TGCTCTCCGAA-3' | GTGATGCG-3' |
GIMAP genes | |||
GIMAP1 | NM_175860 | 5'-TTCAGGGACT | 5'-TTCCTCATCTCT |
| Â | Â | GTCAGCAAGAA-3' | TGCCATCTTC-3' |
GIMAP3 | AF337052 | 5'-GGACTGGCTG | 5'-AGGATCCTCAGT |
| Â | Â | GACCAGAGACT-3' | GGCCTTGAC-3' |
GIMAP4 | NM_174990 | 5'-GAAAGTTCAA | 5'-CCCCAAGGATAC |
| Â | Â | GGAGCAGCCATGA-3' | TGTTCCCTGTT-3' |
GIMAP5 | NM_175035 | 5'-GGAACACCTT | 5'-TCCTCAGGCAGC |
| Â | Â | CAGAAGAGCACAT-3' | TAGATTCTTGT-3' |
GIMAP6 | NM_153175 | 5'-GTGAGCACAG | 5'-ACCAGAAGGAGC |
| Â | Â | ACCTGAGAAGAATC-3' | TGCAGTCTCT-3' |
GIMAP7 | NM_174960 | 5'-AGAGATCCTGAACT | 5'-CATGTCAAGGCA |
| Â | Â | CTCATTAACTGTTG-3' | AGCTGACTCTA-3' |
GIMAP8 | AB178029 | 5'-AGCCTTGCGT | 5'-TCCCCAGGATAG |
| Â | Â | CATCAGAGAGAAA-3' | TGTTCCCAGTT-3' |
GIMAP9 | NM_146167 | 5'-CCTGGTGCAG | 5'-CTTAACGTGGAA |
| Â | Â | TGCTCTCCGAA-3' | GCTCTGGAAT-3' |