Figure 6
Effect of trypsin on p120 and p68. Infected erythrocytes at the trophozoite stage were treated with either SLO (lanes 1, 2, 3) or 0.1% saponin (SAPO) (lanes 4, 5, 6) and separated into supernatants (SNT, lanes 1 and 4) and pellets (P). Pellets were treated (P+T, lanes 3 and 6) or not (P-T, lanes 2 and 5) with 500 μg.ml-1 trypsin for 30 min at room temperature and the reaction was stopped by using trypsin inhibitor (1mg.ml-1) for 5 min on ice before separation of samples by SDS-PAGE. Anti-MAP1 antibodies [6]were used to detect PfA-M1 forms, indicated on the left (A) or PfPV1 (B) and PfAldolase (C) as controls. The amount of p120 relative to p68 was strikingly reduced in SAPO pellets as compared to SLO pellets, after trypsin treatment.