Table 1 Amplification primers and double-labelled hydrolysis probes for PgMET and HumTuBB.
Primer/Probe | 5' Fluorophore | Sequence | 3' Quencher |
|---|---|---|---|
PgMET_F1 | 5'-TGAAAGCAGCGTAGCTCAGA | ||
PgMET_R2 | 5'-CGCGTGGTTTCGATCCACG | ||
PgMET_pB | FAM | 5'-GGGGCTCATAACCCCCAGGA | BHQ2 |
HumTuBB_F2 | 5'-AAGGAGGTCGATGAGCAGAT | ||
HumTuBB_R2 | 5'-GCTGTCTTGACATTGTTGGG | ||
HumTuBB_Joe | JOE | 5'-TTAACGTGCAGAACAAGAACAGCAGCT | BHQ2 |