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Table 2 HGMS-depletion experiments

From: Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells

 

Initial culture:

 

HGMS depleted fraction:

 

Experiment No.

Rings+early trophozoites [%]

Late trophozoites + schizonts [%]

Rings+early trophozoites [%]

Late trophozoites + schizonts

1

5.32

0.38

5.19

none

2

3.54

0.77

3.21

none

3

11.57

1.18

11.86

none

4

4.60

1.68

4.30

none

5

4.67

4.48

4.69

none

  1. For each experiment, 50 μl of packed cells from Plasmodium culture were applied to the depletion column as described in material and methods. The flow-through was collected and analyzed microscopically (1000 rbc counted). Experiments were performed on different days from different cultures.