Glucose-6-phosphate dehydrogenase deficiency among malaria patients of Honduras: a descriptive study of archival blood samples

Table 2 Primer sequences, exons targeted, and restriction enzymes used in the PCR-RFLP assay to detect two G6PD mutations

Variant	Sequence (5´–3´)	Exon	Restriction enzyme	References
A− (202 G>A, 376 A>G)	GTGGCTGTTCCGGGATGGCCTTCTG AGGGCAACGGCAAGCCTTAC	IV, V	NlaIII	[22, 35]
A+ (376 A>G)	CTGCGTTTTCTCCGCCAATC AGGGCAACGGCAAGCCTTAC	V	FokI	[22, 35]

ISSN: 1475-2875