Fig. 5
From: Upstream AUGs and upstream ORFs can regulate the downstream ORF in Plasmodium falciparum
Firefly luciferase readings of the constructs Pf86 and Pf86-60var containing different point mutations. Firefly luciferase readings of the constructs Pf86, Pf86-60var and Pf86-60var(mut 1 to 6), Pf86-60var(mut 2), Pf86-60var(mut 2 to 6), Pf86-60var(mut 1, 3, 4, 5, 6), and Pf86-60var(mut 1 to 5) obtained after transiently transfecting them in parasite. Construct Pf86-stop where the second codon of the luciferase gene in the vector Pf86 was converted to a stop codon was used as a negative control. Firefly luminescence units were normalized against those of Renilla for each construct. Normalized Firefly readings of each construct have been expressed with respect to normalized Firefly readings of Pf86. Firefly luciferase readings and standard deviations have been calculated from five replicates. Normalized firefly readings were in the range of 1000–7000 RLUs, measured in a luminometer