Fig. 1
From: Biochemical and functional characterization of Plasmodium falciparum DNA polymerase δ
SDS–PAGE and western blot analysis of PfPolδ-cat expression. a SDS-PAGE (7.5 %) and b western blot analysis of PfPolδ-cat. Anti-His antibody was used for western blot analysis. Lane M molecular weight markers; lane 1 induced whole cell of E. coli LMG194 carrying pBAD vector with lacZ gene containing His6 sequence; lane 2 non-induced whole cell of E. coli LMG194 carrying pBAD vector; lane 3 non-induced whole cell of E. coli LMG194 carrying PfPolδ-cat vector; lane 4 induced whole cell of E. coli LMG194 carrying PfPolδ-cat vector; lane 5 HisTrap HP column purified protein