Fig. 5
VCAM-1 expression is increased in the lungs of wild type and αDβ2-deficient mice after infection. Wild type and αDβ2-deficient mice were infected with PbA and lungs were harvested at sacrifice at 7 days of infection. Lungs from infected wild type animals at day 0 were also obtained and handled in the same fashion. Staining for VCAM-1 was accomplished with a specific fluorescently labelled rat anti-mouse antibody (red staining). A rat anti-mouse IgG2b was used as an irrelevant control antibody. Nuclei were stained with DAPI. The sections were examined and photographed by immunoflourescent microscopy at ×20 magnification (50 μm). The patterns shown in the panels are representative of those seen in samples from 3 individual animals for each condition