Fig. 5

CD4⁺ T cells exhibit intravascular localization in IL-10^−/− animals infected with P. chabaudi. A 30 μm sagittal section of a female IL-10^−/− (KO) brain 8 days post-inoculation with 10⁵ Pcc-iRBCs i.p. Animals were injected with 2 × 10⁶ CTV⁺ CD4 T cells i.p. 3.5 h before sacrifice, and 40 μg of DyLight488 labelled Lycopersicon esculentum (Tomato) Lectin i.v. 20 min before sacrifice without perfusion. Left panel Confocal image of an adoptively transferred CTV⁺ CD4⁺ T cell located in the Lectin⁺ microvasculature of the KO brain. Right panel Quantitation of adoptively transferred CTV⁺ CD4 T cells found inside or outside Lectin⁺ blood vessels within the KO brain. Imaging performed using a 20× objective with 4× digital zoom to visualize individual T cells within microvasculature