Loci | Primer name | Primer (5′-3′) | PCR conditions | Reference |
---|---|---|---|---|
Pfcrt SNPs at codons 72 and 76 | OF P1 OR P2 | 5′-CCGTTAATAATAAATACACGCG-3′ 5′-CGGATGTTACAAAACTATAGTCC-3′ | 35 cycles of 94 °C for 30 s; 56 °C for 30 s; and 62 °C for 1 min | Mekonnen et al. [11] |
NF P3 NR P4 | 5′-AGGTTCTTGTCTTGGTAAATTTGC-3′ 5′-CAAAACTATAGTTACCAATTTTG-3′ | 30 cycles of 94 °C for 30 s; 56 °C for 30 s; and 65 °C for 1 min | ||
Pfmdr1 SNPs at codon 86 and 184 | OF P5 OR P6 | 5′-AGGTTGAAAAAGAGTTGAAC-3′ 5′-ATGACACCACAAACATAAAT-3′ | 30 cycles of 94 °C for 30 s; 55 °C for 30 s; and 65 °C for 1 min | |
NF P7 NR P8 | 5′-ACAAAAAGAGTACCGCTGAAT-3′ 5′-AAACGCAAGTAATACATAAAGTC-3′ | 30 cycles of 94 °C 30 s; 60 °C for 30 s; and 65 °C for 1 min | ||
Pfmdr1 SNPs at codon 1034,1024, and 1246 | OF P9 OR P10 | 5′-GTGTATTTGCTGTAAGAGCT-3′ 5′-GACATATTAAATAACATGGGTTC-3′ | 34 cycles of 94 °C for 30 s; 55 °C for 1 min and 72 °C for 2 min | |
NF P11 NR 12 | 5′-CAGATGATGAAATGTTTAAAGATC-3′ 5′-TAAATAACATGGGTTCTTGACT-3′ | 29 cycles of 94 °C for 30 s; 60 °C for 30 s; and 65 °C for 1 min | ||
Pfatp6 at codon 243, 263 and 431 | NF P13 NR P14 | 5′-TCATCTACCGCTATTGTATG-3′ 5′-TCCTCTTAGCACCACTCC-3′ | 35 cycles of 94 °C for 45 s; 55 °C for 1 min; 72 °C for 1 min | Zakeri et al. [24] |
Pfatp6 SNPs at codon 623 and 769 | NF P15 NR P16 | 5′-TGGAGACAGTACCGAATTAGC-3′ 5′-TCTTCCTACATATTTACGTGGTG-3′ | ||
Pfk13 | OF P17 OR P18 | 5′-CGGAGTGACCAAATCTGGGA-3′ 5′-GGGAATCTGGTGGTAACAGC-3′ | 30 cycles of 95 °C for 30 s; 58 °C for 2 min; 72 °C for 2 min | Ariey et al. [16] |
NF P19 NR P20 | 5′-GCCAAGCTGCCATTCATTTG-3′ 5′-GCCTTGTTGAAAGAAGCAGA-3′ | 40 cycles of 95 °C for 30 s; 60 °C for 1 min; 72 °C for 1 min |