Proof of concept: used malaria rapid diagnostic tests applied for parallel sequencing for surveillance of molecular markers of anti-malarial resistance in Bissau, Guinea-Bissau during 2014–2017

Table 2 SNP prevalence, mixed infections counted in both groups

Gene	Codon	Amino acid	2014		2015		2016
Gene	Codon	Amino acid	Percentage	Count	Percentage	Count	Percentage	Count
18S				228		676		486
pfcrt	74	M	74	64	67	267	48	120
	74	I	43	37	53	209	61	152
	75	N	74	64	67	267	48	120
	75	E	43	37	53	209	61	152
	76	K	74	64	68	271	48	120
	76	T	43	37	53	210	61	152
pfmdr1	86	N	88	70	97	351	92	202
	86	Y	14	11	4	13	13	29
	184	F	70	56	81	315	74	163
	184	Y	34	27	42	163	38	84
	1034	S	100	67	100	182	100	202
	1034	C	0	0	0	0	0	0
	1042	N	100	67	100	182	100	202
	1042	D	0	0	0	0	0	0
	1246	D	96	64	100	182	100	201
	1246	Y	4	3	0	0	0	1
pfdhfr	51	N	28	24	9	40	16	80
	51	I	76	65	94	408	92	471
	59	C	29	25	11	47	19	96
	59	R	76	65	93	403	91	466
	108	S	20	17	7	35	7	40
	108	N	84	73	96	449	97	526
	164	I	100	86	100	432	100	513
	164	L	0	0	0	0	0	0
pfdhps	436	S	73	36	77	157	68	127
	436	A	31	15	30	62	36	67
	437	A	67	33	57	114	47	88
	437	G	33	16	53	107	58	109
	540	K	98	52	99	134	99	186
	540	E	2	1	1	2	1	2
	581	A	100	53	100	136	100	185
	581	G	0	0	0	0	0	0
	613	A	100	52	98	129	99	187
	613	S/T	0	0	2	2	1	1

ISSN: 1475-2875