Entomological indicators of Plasmodium species transmission in Goma Tsé-Tsé and Madibou districts, in the Republic of Congo

Table 1 Primers using in molecular identification of An. gambiae s.l. in Plasmodium infection

	Primer	Primer sequences	Attending band
Primers	Plas_F	5ʹ-GCTTAGTTACGATTAATAGGAGTAGCTTG-3ʹ
Primers	Plas_R	GAAAATCTAAGAATTTCACCTCTGACA-3ʹ
Probe	Falci +	5ʹ- TCTGAATACGAATGTC-3ʹ	FAM
Probe	OVM +	5ʹ- CTGAATACAAATGCC-3ʹ	HEX
First round PCR	rPLU6	5ʹ-TTA AAA TTG TTG CAG TTA AAA CG-3ʹ
First round PCR	rPLU5	5ʹ-CCT GTT GTT GCC TTA AAC TTC-3ʹ
Second round PCR	rFAL1	5ʹ-TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT-3ʹ	205 bp
	rFAL2	5ʹ-ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC-3ʹ	205 bp
	rMAL1	5ʹ-ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC-3ʹ	105 bp
	rMAL2	5ʹ-AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA-3ʹ	105 bp
	rOVA1	5ʹ-ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA-3ʹ	800 bp
	rOVA2	5ʹ-GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG-3ʹ	800 bp
	rVAV1	5ʹ-GCT TCG GCT TGG AAG TCC-3ʹ	120 bp
	rVAV2	5ʹ-CCG AAT TCA GTC CCA CGT-3ʹ	120 bp
SINE_200	Sine_F	TCG CCT TAG ACC TTG CGT TA	Ag ss = 249 bp Ac = 479, Aa = 223
SINE_200	Sine_R	CGC TTC AAG AAT TCG AGA TAC	Ag ss = 249 bp Ac = 479, Aa = 223
COCTAIL An. funestus group multiplex PCR	ITS2A	5ʹ TGT GAA CTG CAG GAC ACA T 3ʹ
	ITS2B	5ʹ TAT GCT TAA ATT CAG GGG GT 3ʹ
	UV	5ʹ TGT GAA CTG CAG GAC ACA T 3ʹ
	FUN	5ʹ GCA TCG ATG GGT TAA TCA TG 3ʹ	506
	VAN	5ʹ TGT CGA CTT GGT AGC CGA AC 3ʹ	578
	RIV	5ʹ CAA GCC GTT CGA CCC TGA TT 3ʹ	411
	PAR	5ʹ TGC GGT CCC AAG CTA GGT TC 3ʹ	252
	LEES	5ʹ TAC ACG GGC GCC ATG TAG TT 3ʹ	146

ISSN: 1475-2875