Fig. 3
Imaging of P. cynomolgi liver stage schizonts using IFA-CLEM. A Micrographs showing a P. cynomolgi schizont at 7 dpi in a primary NHP hepatocyte stained for UIS4 (green) and nucleic acids (Hoechst, blue) and imaged with fluorescence and phase contrast microscopy. Scale bar is 20 μm. The same schizont is also represented on a TEM micrograph obtained using IFA-CLEM. Scale bar is 5 μm. B TEM micrographs of two other P. cynomolgi schizonts at 7 dpi (i and ii). Scale bars are 5 μm. C–H TEM micrographs showcasing the ultrastructural features of P. cynomolgi liver stage schizonts, including an apicoplast (Api), host glycogen granules (GG), host mitochondria (MH), dense/electron-opaque P. cynomolgi vacuoles (DVPc), large P. cynomolgi vacuoles (LVPc), the P. cynomolgi endoplasmic reticulum (ERPc), the P. cynomolgi mitochondrial network (MPc), P. cynomolgi nuclei (NPc) and the parasitophorous vacuole (PV) in hepatocytes infected with P. cynomolgi schizonts at 7 dpi. Note the host mitochondria positioned in proximity to the PVM in (C). PVM, parasitophorous vacuole membrane; PPM, parasite plasma membrane. Scale bars are 1 μm (C) or 500 nm D–H