Fig. 5
Thermal inactivation analysis of recombinant G6PD variants. Residual enzyme activity was measured after the protein was heated at different temperatures (25 to 65 °C) for 20 min in the presence of various concentrations of NADP+ (0, 10 and 100 μM). T1/2 is the temperature at which the enzyme loses 50% activity. Error bars represent mean ± SD of triplicate measurements