Fig. 7

Susceptibility of recombinant G6PD variants to trypsin digestion. Residual enzymatic activity was measured after incubation with 0.5 mg/mL trypsin at 25 °C for 5 min in the presence of various concentrations of NADP⁺ (0, 10 and 100 μM). Residual enzyme activity was expressed as a percentage of the activity for the same enzyme in the absence of trypsin. Error bars represent the mean ± SD of triplicate measurements