Table 3 Kinetic parameters of recombinant G6PD variants
Construct | Amino acid change | kcat (s–1) | Km G6P (μM) | Km NADP+ (μM) |
|---|---|---|---|---|
WT | – | 293.2 ± 7.0 | 42.1 ± 3.6 | 12.9 ± 3.3 |
Gaohe | His27Arg | 96.6 ± 3.6 | 53.0 ± 6.8 | 11.0 ± 3.4 |
Valladolid | Arg136Cys | 282.3 ± 4.4 | 50.1 ± 2.7 | 8.4 ± 2.6 |
Gond | Met159Ile | 255.5 ± 6.5 | 64.0 ± 5.3 | 19.5 ± 6.5 |
Viangchan | Val298Met | 107.7 ± 7.4 | 58.7 ± 5.0 | 18.0 ± 4.1 |
Canton | Arg459Leu | 64.8 ± 2.6 | 25.5 ± 4.1 | 10.5 ± 3.9 |
Gaohe + Viangchan | His27Arg + Val298Met | 57.0 ± 3.2 | 47.9 ± 7.4 | 10.3 ± 2.2 |
Valladolid + Viangchan | Arg136Cys + Val298Met | 140.3 ± 3.0 | 31.0 ± 2.8 | 8.2 ± 1.4 |
Canton + Viangchan | Arg459Leu + Val298Met | 22.6 ± 1.2 | 17.3 ± 3.6 | 9.6 ± 2.3 |