Let it glow: genetically encoded fluorescent reporters in Plasmodium

Thiele, Pia J.; Mela-Lopez, Raquel; Blandin, Stéphanie A.; Klug, Dennis

doi:10.1186/s12936-024-04936-9

Malaria Journal

Table 1 List of reporter lines in P. berghei with FP expression in an otherwise unaltered genetic background. Compilation of transgenic lines with ectopic expression of FPs either as monomer or in fusion with luciferase

From: Let it glow: genetically encoded fluorescent reporters in Plasmodium

Parasite line	Fluorescent stages	Promoter	FP reporter	Selectable marker	Target site	RMgmDB	References
fluo^α	Complete life cycle oocysts, sporozoites	eEF1α/csp	GFP/mCherry	removed	SIL12	–	[38]
wt_red_230p	Complete life cycle	eEF1α	mCherry	tgDHFR-TS	230p	1227	[89]
Δp230p-GFP^α	Complete life cycle	hsp70	GFP	removed	230p	1026	[44]
Δp230p-GFP-Luc^α	Complete life cycle	eEF1α	GFP-Luciferase	removed	230p	1027	[44]
Δp230p-mCherry^α	Complete life cycle	eEF1α	mCherry	removed	230p	–	[44]
Beryellow	Complete life cycle	hsp70	YFP	hDHFR	SIL6	948	[43]
Berred	Complete life cycle	hsp70	mCherry	hDHFR	SIL6	949	[43]
Bergreen	Complete life cycle	hsp70	GFP	hDHFR	SIL6	947	[43]
404cl1^β	Complete life cycle	eEF1α	GFP	FACS	C-rRNA	–	[36]
507cl1^β	Complete life cycle	eEF1α	GFP	FACS	230p	7	[10]
662cl1*^β	Complete life cycle	eEF1α	GFP-Luciferase	FACS	C-rRNA	–	[10]
PbGFP-LUCcon^β	Complete life cycle	eEF1α	GFP-Luciferase	FACS	230p	29	[10]
PbGFPcon*	Complete life cycle	eEF1α	GFP	tgDHFR-TS	C-rRNA	5	[21]
PbANKA-Cherry 2204cl5^α	Sporozoites, liver stages	uis4	mCherry	removed	230p	-	[51]
PbGFP103464*	Liver stages	lisp2	GFP	tgDHFR-TS	C-/D-rRNA	593	[22]
1037cl1^β	Merozoites, sporozoites	ama1	GFP-Luciferase	FACS	230p	32	[48]
gfp-luc/cl2*	Merozoites, sporozoites	ama1	GFP-Luciferase	tgDHFR-TS	C-/D-rRNA	200	[48]
354cl4*	Merozoites, sporozoites	ama1	GFP-Luciferase	tgDHFR-TS	D-rRNA	30	[49]
28R/GTA^α	Female gametocytes All stages except female gametocytes	P28 α-tubulin II	mRuby mNG	removed	230p	164	[50]
707cl1*	Gametocytes	mdv-1/peg3	GFP	tgDHFR-TS	C-rRNA	251	[14]
820cl1m1cl1^α	Male gametocytes Female gametocytes	mg1 ccp2	GFP RFP	removed	230p	164	[14]
PbFluspo*^γ	Oocysts, sporozoites	csp	GFP	pbDHFR-TS	5’ UTR of csp	77	[52]
mCherry_Hsp70	Complete life cycle	hsp70	mCherry	removed	230p	-	[9]

^* Single crossover integration
α Selection marker was removed through negative selection
β Parasite lines were generated without chemical selection and cloned by fluorescence activated cell sorting (FACS)
γ NK65 genetic background (other lines were made in the genetic background ANKA)

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ISSN: 1475-2875

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