Let it glow: genetically encoded fluorescent reporters in Plasmodium

Thiele, Pia J.; Mela-Lopez, Raquel; Blandin, Stéphanie A.; Klug, Dennis

doi:10.1186/s12936-024-04936-9

Malaria Journal

Table 2 List of reporter lines in P. falciparum with FP expression in an otherwise unaltered genetic background

From: Let it glow: genetically encoded fluorescent reporters in Plasmodium

Parasite line	Fluorescent stages	Promoter	FP reporter	Selectable marker	Target site	Strain	References
GFP@ef1α	Complete life cycle	eEF1α	GFP	Removed	Pfs47	NF54	[25]
mCh-Luc@ef1α	Complete life cycle	eEF1α	mCherry-Luciferase	Removed	Pfs47	NF54	[25]
GFP@ef1α_NF135	Complete lifecycle	eEF1α	GFP	Removed	Pfs47	NF135	[25]
mCh-Luc@sui1	Complete life cycle	sui1	mCherry-Luciferase	Removed	Pfs47	NF54	[25]
GFP@sui1_NF135	Complete life cycle	sui1	GFP	Removed	Pfs47	NF135	[25]
GFP@40 s	Complete life cycle	40S	GFP	Removed	Pfs47	NF54	[25]
mCh-Luc@40 s	Complete life cycle	40S	mCherry-Luciferase	Removed	Pfs47	NF54	[25]
GFP@40s_NF135	Complete life cycle	40S	GFP	Removed	Pfs47	NF135	[25]
Pf-ef1a-tdTomato*	Complete life cycle	eEF1α	tdTomato	BSD	Pfs47	NF54^Pfs47attB	[41]
mCherry-luc@gapdh	Complete life cycle	gapdh	mCherry-Luciferase	Removed	Pfs47	NF54	[47]
GFP@hsp70	Complete life cycle	hsp70	GFP	Removed	230p	NF54	[46]
GFP@cam	Complete life cycle	cam	GFP	Removed	230p	NF54	[46]
GFP@gapdh	Complete life cycle	gapdh	GFP	Removed	230p	NF54	[46]
NF54-HGL	Complete life cycle	hsp70	GFP-Luciferase	Removed	Pfs47	NF54	[45]
NF54HT-GFP–luc*	Complete life cycle	eEF1α	GFP-Luciferase	hDHFR	Pfs47	NF54	[40]
3D7HT-GFP*	Complete life cycle	eEF1α	GFP	hDHFR	Pfs47	3D7	[39]
mCherry-luc@etramp10.3	Liver stages, gametocytes, sporozoites	etramp10.3/peg4	mCherry-Luciferase	Removed	Pfs47	NF54	[47]
Pf-peg4-tdTomato*	Liver stages, gametocytes, sporozoites	etramp10.3/peg4	tdTomato	BSD	Pfs47	NF54^Pfs47attB	[41]
NF54-10.3-Tom^α	Gametocytes	etramp10.3/peg4	tdTomato	hDHFR-yFCU	lisp1	NF54	[26]
E5-10.3-Tom^α	Gametocytes	etramp10.3/peg4	tdTomato	hDHFR-yFCU	lisp1	E5	[26]
NF54-gexp02-Tom^α	Gametocytes	gexp02	tdTomato	hDHFR-yFCU	lisp1	NF54	[26]
E5-gexp02-Tom^α	Gametocytes	gexp02	tdTomato	hDHFR-yFCU	lisp1	E5	[26]
3D7^{α−tubII/GFP}*	Gametocytes	α-tubulin II	GFP	BSD	3’hrp2	3D7	[90]
NF54^pfs16*	Gametocytes	Pfs16	GFP-Luciferase	Removed	cg6	NF54^attB	[34]
NF54^pfs48/45*	Gametocytes	Pfs48/45	GFP-Luciferase	Removed	cg6	NF54^attB	[34]
NF54^mal8p1.16*	Gametocytes	Mal8p1.16	GFP-Luciferase	Removed	cg6	NF54^attB	[34]
Dd2-GFP*	Complete life cycle	hsp70	GFP	hDHFR	cg6	Dd2	[91]
Pfpare	Intraerythrocytic cycle	Pfpare	mNG	BSD	Pfpare	Dd2	[28]
PfOCB13-GFP	Complete life cycle	hsp70	GFP	Free	OBC13	NF54	[29]
GFP-NanoLuc	Complete life cycle	40S	GFP-T2A-NanoLuc	Free	Pfs47	NF54	[12]

Compilation of transgenic lines with ectopic expression of FPs either as monomer or in fusion with luciferase
^* Single crossover integration
α Parasite line potentially has a phenotype in the liver stage delaying egress of merozoites

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