Fig. 5

Infectivity of SPZ to HC-04.j7 hepatocytes. A Representative image of overlay 3 × 3 fields by ImageXpress of liver schizonts in HC-04.j7 cells, 72 h post infection with day 14, 17 and 20 SPZ. HC-04.j7 nucleus in blue, liver schizont double positive, HSP70 in green, GAPDH in red and overlay in yellow. B Number of HSP70 and GAPDH expressing exoerythrocytic forms (EEFs) 72 h after infection with day 14 SPZ (light blue), day 17 SPZ (blue) and day 20 SPZ (dark blue). C 18S PCR of infection assay of HCO4.j7 cells infected with SPZ at day 14 (light blue), day 17 (blue) and day 20 (dark blue). D Exp1 PCR of infection assay with HCO4.j7 cells. Analysis using Mann Whitney U test. Individual experiments indicated with different shapes (circle, square or triangle). *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005 and ****p ≤ 0.0001