Figure 3From: Dual fluorescent labelling of the human malaria parasite Plasmodium falciparum for the analysis of the ABC type transporter pfmdr2Time dependent Cd accumulation in RBCs infected with P. falciparum Cd resistant and sensitive strains. (A) Fluo-3/AM fluorescence in a time dependent manner analysed by flow cytometry. After gating the populations for positive and negative to Hoechst, Fluo-3/AM fluorescence geometric mean was calculated. The samples without Cd served as control. Sensitive and resistant denote RBCs infected with sensitive and resistant parasite respectively. RBC-s and RBC-r denote non-infected RBCs originating from the sensitive and resistant cultures respectively. Each time point is a mean of duplicates, Bars ± SD. (B) Confocal images: I: Hoechst (Blue), II: Fluo-3/AM (Green), III: combined Hoechst and Fluo-3/AM, IV: combined Hoechst and Fluo-3/AM with DIC. [Red scale bar = 20μm]. The results are from one representative experiment of two performed. For the sensitive and resistant strains parasitaemia was 10%.Back to article page