Gene Primer pair | Sequence 5' ⇒ 3' | PCR product size (bp) | PCR reaction conditions |
---|---|---|---|
pfATPase6 | |||
   atp6-1F | tcatctaccgctattgtatgtgg | 777 | 94oC 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 40''); 72°C 5' |
   atp6-1R | attcctcttagcaccactcct | ||
   atp6-2F | tcaccaaggggtatcaacaa | 692 | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 40''); 72°C 5' |
   atp6-2R | tggcataatctaattgctcttcc | ||
   atp6-3F | atgtatagctgttgtaatcaacctaga | 822 | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 40'); 72°C 5' |
   atp6-3R | tcactatatggatcagcttcatca | ||
   atp6-4F | ccagtacattgaatgaaaatg | 605 | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 40''); 72°C 5' |
   atp6-4R | acgtggtggatcaataatacct | ||
pftctp | |||
   pftctp-1F | atgaaagtatttaaagacgtt | 462 | 94°C 5' followed by 40 cycles (94°C 15''; |
   pftctp-1R | ttcttctcctttataataagaat | 50°C 30''; 72°C 40''); 72°C 5' | |
pfmdr1 | |||
   mdr1-1F | tgaacaaaaagagtaccgctga | 823 | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 1'); 72°C 5' |
   mdr1-1R | ccataccaaaaaccgaatgc | ||
   mdr1-2F | caagcggagtttttgcattt | 1062 | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 30''; 72°C 1'); 72°C 5' |
   mdr1-2R | ttctctgtttttgtccacctga | ||
pfcrt | |||
   pfcrt-1'F | atggctcacgtttaggtgga | 94°C 5' followed by 40 cycles (94°C 15''; 55°C 15''; 72°C 40''); 72°C 2' | |
   pfcrt-2R | aaagcttcggtgtcgttc | ||
   pfcrt-1F | tgtgctcatgtgtttaaactt | 94°C 5' followed by 25 cycles (94°C 15''; 48°C 30''; 72°C 20''); 72°C 5' | |
   pfcrt-2'R | ggaatagattctcttataaatcc | 282 |