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Table 2 P. falciparum CSP-ELISA results and confirmation by Plasmodium spp. specific PCR

From: False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination

  Cambodia Vietnam
  N° Tested N° ELISA + N° PCR confirmed N° Tested N° ELISA + N° PCR confirmed
Anthropophilic 1144 12 11 864 12 8
An. dirus s.l. 1144 12 11 1 864 12 8 1
Zoophilic trend 8089 104 0 (+1)* 6063 48 1
An. barbirostris 1628 33 0 (+ 1)* na na na
An. jamesii 17 1 0 na na na
An. maculatus s.l. 3596 50 0 5081 42 0
An. minimus s.l. 2848 20 0 530 4 1 2
An. pampanai na na na 65 1 0
An. splendidus na na na 387 1 0
  1. The distinction into anthropophilic and zoophilic trend is made based on the results presented in Trung et al[31].
  2. * The +1 mentioned between brackets means that the Plasmodium spp. specific PCR was positive, but cloning and subsequent sequencing of the PCR product revealed the presence of P. malariae instead of P. falciparum.
  3. 1 All PCR confirmed specimens were molecularly identified as An. dirus s.s.
  4. 2 The PCR confirmed specimen was molecularly identified as An. minimus s.s.
  5. na = not applicable.