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Table 2 P. falciparum CSP-ELISA results and confirmation by Plasmodium spp. specific PCR

From: False positive circumsporozoite protein ELISA: a challenge for the estimation of the entomological inoculation rate of malaria and for vector incrimination

 

Cambodia

Vietnam

 

N° Tested

N° ELISA +

N° PCR confirmed

N° Tested

N° ELISA +

N° PCR confirmed

Anthropophilic

1144

12

11

864

12

8

An. dirus s.l.

1144

12

11 1

864

12

8 1

Zoophilic trend

8089

104

0 (+1)*

6063

48

1

An. barbirostris

1628

33

0 (+ 1)*

na

na

na

An. jamesii

17

1

0

na

na

na

An. maculatus s.l.

3596

50

0

5081

42

0

An. minimus s.l.

2848

20

0

530

4

1 2

An. pampanai

na

na

na

65

1

0

An. splendidus

na

na

na

387

1

0

  1. The distinction into anthropophilic and zoophilic trend is made based on the results presented in Trung et al[31].
  2. * The +1 mentioned between brackets means that the Plasmodium spp. specific PCR was positive, but cloning and subsequent sequencing of the PCR product revealed the presence of P. malariae instead of P. falciparum.
  3. 1 All PCR confirmed specimens were molecularly identified as An. dirus s.s.
  4. 2 The PCR confirmed specimen was molecularly identified as An. minimus s.s.
  5. na = not applicable.