Cross-reactivity of anti-VAR2CSA on a panel of CSA-binding parasites. A) Flow cytometry analysis of rat anti-VAR2CSA plasma on the homologous 7G8-CSA parasite line. Preimmune plasma is shown in grey, black line for rat #1, grey for rat #2, and dashed line for rat #3. The reactivity of protein control, anti-IT4var22 DBL3 plasma is shown as well. B) Summary of homologous surface reactivity against 7G8-CSA and IT4-CSA parasite lines. Each dot represents the mean adjusted MFI from duplicate experiments observed for each animal.