Plasmodium vivax lineages: geographical distribution, tandem repeat polymorphism, and phylogenetic relationship

Malaria Journal

Table 1 Plasmodium vivax primers list and their annealing temperature

Gene/locus	Primer name	Primer sequences (5'-3')	Annealing Tm (°C)
18S SSU rRNA	SSU-F	ATGAACGAGATCTTAACCTGC	65/55
	SSU-R	CATCACGATATGTA5TGATAAAGATTACC
MiniSat-1	Mini1-F	ATGCTTCATTGGGTCCAC	50
	Mini1-R	TCGAACAGGACAATGCTG
MiniSat-2	Mini2-F	TCACCGGTGGGTCCTTCG	50
	Mini2-R	GCAGCGACGAACCGTCAC
MiniSat-5	Mini5-F	CAACCTGCAGAGCAATGC	55
	Mini5-R	ACGTTTCTGGGCGACTTC
MiniSat-6	Mini6-F	TTGTGCTGTGCTGTGCTG	55
	Mini6-R	ACGGTTGGTATGGTCAGG
MiniSat-8	Mini8-F	AGCCACAATCCCAACTGC	52
	Mini8-R	TGGTGGTTGTGACTCTAG
MiniSat-11	Mini11-F	GGCACAGTGATCATATTCG	55
	Mini11-R	GCGGGTACATAACGCATG
MiniSat-13	Mini13-F	GGCACATGAACTTTTCGG	52
	Mini13-R	TTCACCATGGTCCCTTCG
MiniSat-14	Mini14-F	CTCTTCGTCGCGTCCAGG	52
	Mini14-R	CAGGGTATCCACGACCAG
MiniSat-16	Mini16-F	TATGTACTACCTCCACCC	52
	Mini16-R	AGCGCGAATATGCATACG
PvCDPK	CDPK-F	CGCCTCTTTTTCGAGCCC	55
	CDPK-R	CTGCGCCTTCCGCGTCTT

High-resolution metaphor agarose (3-4% gel) was used for mini-satellite allele sizing. A fixed electrophoresis protocol (2 h @ 80 V) was used for all experiments for consistency in allele sizing. A 20 bp DNA ladder and Genetool software were used (SynGene Inc., UK) for allele sizing.

ISSN: 1475-2875