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Table 2 Proteins identified by differential 2-D DIGE analysis following hyperoxia exposure of P. falciparum

From: Global response of Plasmodium falciparum to hyperoxia: a combined transcriptomic and proteomic approach

gi numberc Gene ID Protein name MW (kDa) pI Master spot number Significance (Mascot score) Average ratio t-Test
P. falciparum
Translation
gi|124512420 MAL8P1.69 14-3-3 protein homologue 29.86 4.96 3289 425 -1.54 0.0078
gi|124513850 PF13_0304 elongation factor 1 alpha 49.16 9.12 2047 130 1.60 0.0090
gi|8918238 PF14_0486 elongation factor 2 85.03 6.30 1030 67 1.72 0.0070
gi|124810293 PF14_0655 RNA helicase-1 45.62 5.48 2268 105 1.53 0.040
Parasitophorous vacuolar membrane Transporter
gi|124810348 PF14_0678 exported protein 2 33.62 5.10 3061 88 -1.92 0.00093
      3062 180 -2.06 0.0046
Glycolysis
gi|124809201 PF14_0341 glucose-6-phosphate isomerase 67.61 6.78 1579 36 -1.63 0.00092
gi|124810131 PF14_0598 glyceraldehyde-3-phosphate dehydrogenase 37.08 7.59 2867 695 -1.69 0.033
Chaperone-assisted protein folding
gi|124512406 PF08_0054 heat shock protein 70 74.39 5.51 1314 257 1.65 0.03
      1326 137 1.58 0.026
gi|505340 PF07_0029 heat shock protein 86 86.77 4.91 1028 412 1.50 0.0052
Amino acids metabolism
gi|86170756 PFF0435w ornithine aminotransferase 47 6.47 2217 96 -2.14 0.0081
gi|124513590 MAL13P1.214 phosphoethanolamine 31.31 5.43 3347 145 -2.05 0.0055
   N-methyltransferase    3372 252 -1.96 0.00089
      3379 253 -2.17 0.00058
      3503 52 -1.62 0.00056
      3385 452 -2.71 0.00026
Proteasome-mediated proteolysis
gi|124512686 MAL8P1.142 proteasome beta-subunit 31.08 6.00 3216 58 1.78 0.015
gi|124513790 MAL13P1.270 proteasome subunit 27.50 6.17 3358 142 1.55 0.049
Homo sapiens
Oxygen transporter
gi|183817   Beta-globin 19.21 6.28 3451 165 7.71 0.020
      3576 102 1.91 0.011
Antioxidant metabolism
gi|4502517   carbonic anhydrase I 28.91 6.59 3431 173 4.18 0.0095
      3444 155 3.47 0.0019
      3455 224 4.02 0.00038
gi|4557014   catalase 59.95 6.9 1648 200 6.47 0.012
gi|16306550   selenium binding protein 1 52.93 5.93 2451 73 -1.64 0.041
gi|168985379   flotillin 1 39.81 6.03 2156 157 1.64 0.040
Glycolysis
gi|31645   glyceraldehyde-3- 36.20 8.26 2804 199 -1.94 0.0042
   phosphate dehydrogenase    2806 181 -1.99 0.012
      2807 132 -1.84 0.0069
      2853 112 -2.17 0.0027
      2854 122 -2.25 0.0032
      2856 96 -2.20 0.0019
  1. The proteins were identified by mass spectrometry following in gel trypsin digestion. The spot numbers correspond to the same numbers in figure 4. The Mascot gi number of the spots, their gene ID (PlasmoDB), their name, the theoretical MW and pI values, as well as the corresponding Mascot score are listed for MS/MS analysis (scores greater than 29 for P. falciparum and 38 for Homo sapiens are considered significant (p0.05)). Paired average volume ratio (hyperoxic versus normoxic conditions) and p-values (t-Test) were obtained using Decyder software. MW: molecular weight.