Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR

Malaria Journal

Table 2 Optimization of RDT fragment sampling and DNA extraction method

1) Selection of RDT fragment
	Proximal nitrocellulose	Distal nitrocellulose	Filter paper
OptiMal (Qiagen method)	27.57	0	39.09
	Proximal nitrocellulose	Conjugate pad	Filter paper
SDFK60 (Qiagen method)	30.55*	38.62	38.62
2) Selection of DNA extraction method
	Elution method	Qiagen method
OptiMal (nitrocellulose strip)	28.59	32.64
SDFK60 (nitrocellulose strip)	31.53	36.12

Cycle threshold (Ct)-values in bold indicate best PCR results (i.e. lowest Ct-values)
* fragment sampling of the nitrocellulose strip together with the conjugate pad gave a Ct-value of 29.28

ISSN: 1475-2875