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Table 5 Detection of mixed infection by PCR on whole blood, PCR on RDT and antigen detection.

From: Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR

  

OptiMAL

SDFK60

 

PCR on whole blood

PCR on RDT

Antigen detection

PCR on RDT

Antigen detection

Mixed infection

Ct-value

Ct-value

Test line(s)

Ct-value

Test line(s)

P. falciparum +P. malariae

36.06 + 26.50

38.23 + 30.13

Pan pLDH

0.00 + 31.17

negative

P. falciparum +P. malariae

29.68 +33.63

32.33 + 39.12

Pf + pan pLDH

34.46 + 37.93

HRP-2

P. falciparum +P. malariae

25.88 + 37.06

27.77 + 39.65

Pfmx

29.97 + 0.00

HRP-2 + pLDH

P. falciparum +P. ovale

36.43 + 25.54

0.00 + 28.11

negative

40.36 + 30.33

pLDH

P. falciparum +P. ovale

28.40 + 37.48

30.19 + 39.60

Pfmx

30.25 + 0.00

HRP-2

  1. Ct-values in bold represent PCR signals obtained for the major Plasmodium species. A negative PCR result (Ct-value = 0.00) indicates a missed minor species.