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Table 5 Detection of mixed infection by PCR on whole blood, PCR on RDT and antigen detection.

From: Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR

   OptiMAL SDFK60
  PCR on whole blood PCR on RDT Antigen detection PCR on RDT Antigen detection
Mixed infection Ct-value Ct-value Test line(s) Ct-value Test line(s)
P. falciparum +P. malariae 36.06 + 26.50 38.23 + 30.13 Pan pLDH 0.00 + 31.17 negative
P. falciparum +P. malariae 29.68 +33.63 32.33 + 39.12 Pf + pan pLDH 34.46 + 37.93 HRP-2
P. falciparum +P. malariae 25.88 + 37.06 27.77 + 39.65 Pfmx 29.97 + 0.00 HRP-2 + pLDH
P. falciparum +P. ovale 36.43 + 25.54 0.00 + 28.11 negative 40.36 + 30.33 pLDH
P. falciparum +P. ovale 28.40 + 37.48 30.19 + 39.60 Pfmx 30.25 + 0.00 HRP-2
  1. Ct-values in bold represent PCR signals obtained for the major Plasmodium species. A negative PCR result (Ct-value = 0.00) indicates a missed minor species.