Figure 6
From: Plasmodium berghei ANKA infection increases Foxp3, IL-10 and IL-2 in CXCL-10 deficient C57BL/6 mice
(A-C): IL-10 production in P. berghei antigen re-stimulation of CD4+CD25+ and CD4+CD25- T cells from CXCL-10-/- and wild type C57BL/6 mice. Day 4 infected mice (n = 6) spleens were harvested and peripheral blood mononuclear cells were isolated. Regulatory T cells (CD4+CD25+) and regulatory T cells-depleted T cells (CD4+CD25-) were isolated using the Miltenyi Biotec Inc (CA, USA) isolation kit. Cells (5 × 105cells/well) were re-stimulated with P. berghei antigens (Ag) (50 μg/ml) plus PMA and ionomycin. Controls consisted of cells re-stimulated with supernatant from uninfected red blood cells (Uninf.). Cells were cultured for 5 days and IL-10 in harvested supernatants was determined by ELISA. There was significant difference in IL-10 production between antigen (Ag) restimulated cells than in controls (Uninf) (Figures 6A and 6B). Interestingly there was no significant difference in IL-10 levels between antigen (Ag) restimulated cells from CXCL-10-/- and WT (Figure 6C).