Figure 4
Histological and TUNEL analysis of mouse brains, sampled according to Figure 1, scheme 4. (A) Number of perivascular and interstitial haemorrhages in two 5 μm serial sections per mouse, in each group from Figure 1, scheme 4. (D0CT = control group at day 0; D0AVA = group with atorvastatin at day 0; D5CT = control group at day 5; D5AVA = group with atorvastatin at day 5; D5MQ = group with mefloquine at day 5; D5MAAVA = group with MQ + AVA at day 5; D8CT = control group at day 8; D8AVA = group with atorvastatin at day 8; D8MQ = group with mefloquine at day 8; D8MQAVA = group with MQ + AVA at day 8; D14MQ = group with mefloquine at day 14; D14MQAVA = group with MQ + AVA at day 14). Significant differences between D5CT and D5MQ, D8CT and D8MQ, D8CT and D8MQAVA, and D14MQ and D14MQAVA. (B) HES staining. Petechial haemorrhage 400×. (C) Perivascular haemorrhage 1000×. (D) Interstitial haemorrhage area with haemozoin pigment 400×. (E) Subarachnoid haemorrhage. (F) Cerebellum perivascular and interstitial haemorrhage 400×. (G) Vascular plugging by monocytes/macrophages (sometimes pigment-containing) with perivascular oedema and few extravasated mononuclear cells 1,000×. (H) Number of apoptotic nuclei in one section per mouse from each group of Figure 1, scheme 4; TUNEL + staining (brown colour with DAB). (I) TUNEL + neurons revealing apoptotic nuclei; 1000×. (J) Focal cerebellar area stained with TUNEL + and showing an interstitial haemorrhage (arrow); 200×. (D0CT = control group at day 0; D0AVA = group with atorvastatin at day 0; D2CT = control group at day 2; D2AVA = group with atorvastatin at day 2; D2MQ = group with mefloquine at day 2; D2MQAVA = group with MQ + AVA at day 2; D5CT = control group at day 5; D5AVA = group with atorvastatin at day 5; D5MA = group with mefloquine at day 5; D5MQAVA = group with MQ + AVA at day 5; D8CT = control group at day 8; D8AVA = group with atorvastatin at day 8; D8MQ = group with mefloquine at day 8; D8MQAVA = group with MQ + AVA at day 8; D14MQ = group with mefloquine at day 14; D14MQAVA = group with MQ + AVA at day 14).