Mosquito bisection as a variable in estimates of PCR-derived malaria sporozoite rates

Malaria Journal

Table 1 PCR results for malaria parasites in mosquitoes from the ‘Humid’ treatment

Infection stage (bisection position)	Numbers				Percent
	Anterior		Posterior		Anterior		Posterior
	+	-	+	-	+	-	+	-
Control (B)	0	27	0	27	0	100	0	100
Oos only (C)	1*	25	0	26	3.8*	96.2	0	100
Oos only (B)	0	27	0	27	0	100	0	100
Oos only (A)	0	7	2	5	0	100	28.6	71.4
Spz + Oos (B)	2	25	0	27	7.4	92.6	0	100

* Corresponding abdomen was not positive.
Numbers of specimens and percentages positive or negative for Plasmodium falciparum, as determined by PCR, for the ‘ Humid’ treatment, for anterior and posterior portions of specimens of Anopheles stephensi, at different stages of infection (Oos only = oocysts only, 6–7 days p.i.; Spz + Oos = sporozoites and oocysts, 14 days p.i.), and different bisection locations (A, B, C, see Figure 1).

ISSN: 1475-2875