Figure 3
Quantification of haemozoin in tissues. C57BL/6 J mice were infected with 104Pb ANKA, Pb NK65 or Pc AS parasites or were left uninfected (Con). At the indicated time intervals after infection, mice were dissected after heart puncture and perfusion. Extracted Hz from 30 – 60 mg tissue of livers (A), spleens (B), lungs (C) and kidneys (D), from half brains (E) and from whole hearts (F) were quantified by haem-enhanced luminescence and expressed as nmol haematin/organ (liver, spleen, lungs and kidneys) or pmol haematin/organ (brain and heart). Each group consisted of 15 to 20 mice, with each dot indicating individual data points. Horizontal dashed lines were used to denote the accuracy limit of the assay for each organ separately. Horizontal bars represent group medians and horizontal lines with asterisks on top indicate statistical comparisons between groups. Asterisks on top of data sets indicate statistical significances compared with the uninfected control group. * p < 0.05, ** p < 0.01 and *** p < 0.001