Skip to main content

Table 1 PCR primers used for amplification of Pfs25 and Pvs25

From: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

Gene target

Primers

Sequences (5′ → 3′)

Nucleotide positions

Product size (bp)

Primary PCR

    

Pfs25 and Pvs25

FV25F0

GAAGATACATGTGAAGAAAAA

237–257* or 163–183#

264 for P. falciparum

 

FV25R0

ATTGGGAACTTTGCCAATA

482–500* or 414–432#

270 for P. vivax

Secondary PCR

    

Pfs25

F25F1

AAATGTGACGAAAAGACTG

264–281*

201

 

F25R1

AGTTTTAACAGGATTGCTTGTATC

441–464*

 

Pvs25

V25F1

ACCCTAGGCAAAGCATG

202–218#

115

 

V25R1

CAAGTGTCTTCCTTCAAAGT

298–317#

 
  1. * and # after GenBankTM accession numbers X07802 and GU256271 for Pfs25 and Pvs25, respectively.