Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

Table 1 PCR primers used for amplification of Pfs25 and Pvs25

Gene target	Primers	Sequences (5′ → 3′)	Nucleotide positions	Product size (bp)
Primary PCR
Pfs25 and Pvs25	FV25F0	GAAGATACATGTGAAGAAAAA	237–257* or 163–183#	264 for P. falciparum
	FV25R0	ATTGGGAACTTTGCCAATA	482–500* or 414–432#	270 for P. vivax
Secondary PCR
Pfs25	F25F1	AAATGTGACGAAAAGACTG	264–281*	201
	F25R1	AGTTTTAACAGGATTGCTTGTATC	441–464*
Pvs25	V25F1	ACCCTAGGCAAAGCATG	202–218#	115
	V25R1	CAAGTGTCTTCCTTCAAAGT	298–317#

* and # after GenBank^TM accession numbers X07802 and GU256271 for Pfs25 and Pvs25, respectively.

ISSN: 1475-2875