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Table 3 Microscopy and molecular diagnosis of malaria species and gametocytes in 235 clinical isolates

From: Simultaneous detection of Plasmodium vivax and Plasmodium falciparum gametocytes in clinical isolates by multiplex-nested RT-PCR

Infection Microscopy 18S rRNA PCR Single nested RT-PCR Multiplex nested RT-PCR
    Pfs25 Pvs25 Pfs25 Pvs25
Monoinfection       
P. falciparum 76 75
Gametocyte 8 68 67
P. vivax 80 78
Gametocyte 30 74 74
Co-infections       
P. falciparum and P. vivax 1 10
Gametocyte 0 9 6 8 6
P. vivax and P. malariae 0 1
Gametocyte 0 0 1 0 1
P. falciparum, P. vivax 0 1
and P. malariae       
Gametocyte 0 0 1 0 1
Total malaria positive cases 157 165
Total P. falciparum 77 86
Gametocyte (%)* 8 (8.9) 77 (89.5) 75 (87.2)
Total P. vivax 81 90
Gametocyte (%)** 30 (31.1) 82 (91.1) 82 (91.1)
  1. * Percentage of P. falciparum gametocyte positives per total P. falciparum positives by 18S rRNA PCR.
  2. ** Percentage of P. vivax gametocyte positives per total P. vivax positives by 18S rRNA PCR.
  3. Dashes indicate no applicable data.