Table 3 Microscopy and molecular diagnosis of malaria species and gametocytes in 235 clinical isolates
Infection | Microscopy | 18S rRNA PCR | Single nested RT-PCR | Multiplex nested RT-PCR | ||
|---|---|---|---|---|---|---|
| Â | Â | Â | Pfs25 | Pvs25 | Pfs25 | Pvs25 |
Monoinfection | Â | Â | Â | Â | Â | Â |
P. falciparum | 76 | 75 | − | − | − | − |
Gametocyte | 8 | − | 68 | − | 67 | − |
P. vivax | 80 | 78 | − | − | − | − |
Gametocyte | 30 | − | − | 74 | − | 74 |
Co-infections | Â | Â | Â | Â | Â | Â |
P. falciparum and P. vivax | 1 | 10 | − | − | − | − |
Gametocyte | 0 | − | 9 | 6 | 8 | 6 |
P. vivax and P. malariae | 0 | 1 | − | − | − | − |
Gametocyte | 0 | − | 0 | 1 | 0 | 1 |
P. falciparum, P. vivax | 0 | 1 | − | − | − | − |
and P. malariae | Â | Â | Â | Â | Â | Â |
Gametocyte | 0 | − | 0 | 1 | 0 | 1 |
Total malaria positive cases | 157 | 165 | − | − | − | − |
Total P. falciparum | 77 | 86 | − | − | − | − |
Gametocyte (%)* | 8 (8.9) | − | 77 (89.5) | − | 75 (87.2) | − |
Total P. vivax | 81 | 90 | − | − | − | − |
Gametocyte (%)** | 30 (31.1) | − | − | 82 (91.1) | − | 82 (91.1) |