Figure 2
From: Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients
Expression of NF-κB p65 in the PBMCs of malaria patients by immunofluorescence method. (A) Representative immunofluorescence images of NF-κB p65 in the PBMCs of malaria patients on admission (a, b, c) and on day 7 post-treatment (d, e, f) compared with healthy controls (HC) as evidenced by cytoplasmic NF-κB p65 staining (arrows) (g) positive control (PC) showing nuclear NF-κB p65 staining (arrows) after stimulation with 50 ng/ml of TNF for 30 min (h) and negative control (NC) by omission of primary antibody (i). Bars = 10 μm. (B) Quantitative analysis of the NF-κB p65 activation in PBMCs of malaria patients. *Significance of p < 0.05 compared with healthy controls (Student t-test),†Significance of p < 0.05 compared with the day of admission (Student t-test). Data are presented as a mean ± SEM.