Development of a TaqMan Allelic Discrimination Assay for detection of Single Nucleotides Polymorphisms associated with anti-malarial drug resistance

Kamau, Edwin; Alemayehu, Saba; Feghali, Karla C; Tolbert, LaDonna S; Ogutu, Bernard; Ockenhouse, Christian F

doi:10.1186/1475-2875-11-23

Table 1 Primers and probe sequences of the SNP assays.

From: Development of a TaqMan Allelic Discrimination Assay for detection of Single Nucleotides Polymorphisms associated with anti-malarial drug resistance

SNP Assay Name	Primers/Probes	Sequence (5'-3')
DHFR16	Forward	CAAGTCTGCGACGTTTTCGATATTT
	Reverse	CCTCATTTTTTTTCCCCTCATTTTTGC
	Probe1	CCTTACAACATGCACATAT
	Probe2	AACCTTACAACATACACATAT
DHFR22	Forward	GCGACGTTTTCGATATTTATGCCATA
	Reverse	TCCTAGACCTCTAAATGTGTAGTTATTAAAAACCT
	Probe1	CCCTCATTTTTGCTTTCAA
	Probe2	CCCTCATTTTTGTTTTCAA
DHFR51	Forward	ACTACACATTTAGAGGTCTAGGAAATAAAGGA
	Reverse	GTTGTAACTGCACAAAAATATTTCATATCTAGGG
	Probe1	CCATGGAAATGTAATACCAT
	Probe2	CCATGGAAATGTATTACCAT
DHFR59	Forward	CTAGGAAATAAAGGAGTATTACCATGGAAATGT
	Reverse	CATCTCTTATATTTCAATTTTTCATATTTTGATTCATTCA
	Probe1	CCCTCATTTTTGCTTTCAA
	Probe2	CCCTCATTTTTGTTTTCAA
DHFR108	Forward	ATGTAAATGATATGCCTAATTCTAAAAAATTACAAAATGT
	Reverse	GACAATATAACATTTATCCTATTGCTTAAAGGT
	Probe1	CTTTCCCAGCTTGTTCT
	Probe2	CTTTCCCAGTTTGTTCT
DHFR164	Forward	ACAAAGTTGAAGATCTAATAGTTTTACTTGGGAAA
	Reverse	TTAATTTCTTTTCTAAAAATTCTTGATAAACAACG
	Probe1	CCCTCATTTTTGCTTTCAA
	Probe2	CCCTCATTTTTGTTTTCAA
MDR86	Forward	AGGAGGAACATTACCTTTTTTTATATCTGTGT
	Reverse	ATTGTACTAAACCTATAGATACTAATGATAATATTATAGGAT
	Probe1	CATCACCTAAATTCATGTTC
	Probe2	CATCACCTAAATACATGTTC
MDR184	Forward	GGTACGAAATTTATAACAATTTTTACATATGCCAGTT
	Reverse	AAAAACGCAAGTAATACATAAAGTCAAACGT
	Probe1	CCTTTTTAGGTTTATATATTTG
	Probe2	CCTTTTTAGGTTTATTTATTTG
MDR1034	Forward	GACAAAAAAGAAGAATTATTGTAAATGCAGCTT
	Reverse	AGGATCCAAACCAATAGGCAAAACT
	Probe1	CTTTGACTGAATCCC
	Probe2	TTTGACAGAATCCC
MDR1042	Forward	GGATTCAGTCAAAGCGCTCAATTA
	Reverse	GTACCTCTTTTAATTAAGAAGGATCCAAACCA
	Probe1	ATAGGCAAAACTATTAATAAA
	Probe2	TAGGCAAAACTATCAATAAA
DHPS581	Forward	TTCTTGTATTAAATGGAATACCTCGTTATAGGAT
	Reverse	TATACATGTATATTTTGTAAGAGTTTAATAGATTGATCATG
	Probe1	TTTCTTCGCAAATCC
	Probe2	TTTCTTCCCAAATCC

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ISSN: 1475-2875

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