Table 2 Primer sequences used in generation of plasmid DNAs.
Plasmid generated | Sequence (5'-3') | PCR fragment size | |
|---|---|---|---|
16-3D7/7G8 | Forward Primer | CAAGTCTGCGACGTTTTCGATATTT | Â |
| Â | Reverse Primer | TTAATTTCTTTTCTAAAAATTCTTGATAAACAACG | 526 bp |
86-K1/7G8 | Forward Primer | TGGGTAAAGAGCAGAAAGAGAAA | Â |
| Â | Reverse Primer | TTGCAACAGTTCTTATTCCCATT | 735 bp |
1034-K1/7G8 | Forward Primer | CAAGCGGAGTTTTTGCATTT | Â |
| Â | Reverse Primer | TTCCACCATCATCTCTTACATCA | 447 bp |
581-K1/7G8 | Forward Primer | TGCATAAAAGAGGAAATCCACA | Â |
| Â | Reverse Primer | TCCAATTGTGTGATTTGTCCA | 357 bp |